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Trends in the prevalence, awareness, treatment, and control of hypertension among young adults in the United States, 1999 to 2014

The libraries were further quantified on a Qubit 2.0 fluorometer using Qubit dsDNA HS kit (Life Technologies, USA) and by quantitative PCR (qPCR) using KAPA SYBR FAST qPCR Master mix and Illumina standards and primer premix (KAPA Biosystems, Wilmington, MA, USA), following the Illumina-suggested protocol

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Sananga and Raph Sananga is often used alongside Raph in traditional Amazonian practices