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Tobacco samples were then pulverized by ceramic mortar and five grams of tobacco powder of each brand sample was added to 25 mL of concentrated HNO 3 , then mixed well and put on a hotplate for 3 hours at 120 o C 12

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It was hypothesised that when WT p53 expression is introduced into tumour cells, the WT p53 proteins will be activated and stabilised due to stress stimuli, such as oncogene expression, hyperproliferative signals and DNA damage