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Operation of the Chromium Controller and library preparation were performed as described by the manufacturer using the Chromium Single Cell A Chip Kit, Chromium Single Cell 3 Library & Gel Bead Kits v2 and Chromium i7 Multiplex Kit (10x Genomics, Pleasanton, CA, USA)

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This includes the likes of nicotine (a very small portion of any e-liquid), flavourings, sweeteners or sometimes even sugar, and the most important components Vegetable Glycerine (VG) and Propylene Glycol (PG)