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Supplementary Figure 12 Analysis of microbiome composition in tobacco-product samples

doi: 10.1007/s00267-015-0517-x 41 FrancisR

A tight close-up shows Bailey with tears in his eyes as he holds his head in his hands and begins to pray: God, God, dear Father in Heaven

The genomic DNA was isolated from the spermatozoa using the QIAamp DNA Mini Kit, followed by mitochondrial DNA amplification via the REPLI-g Mitochondrial DNA Kit supplied by QIAGEN (Hilden, Germany), adhering to the kits instruction manual

The results indicate that the lower the tar, nicotine, and carbon monoxide amounts, as well as the longer the cigarette filter, the lower are the PM levels