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We discussed different non-invasive methods such as serum, fecal, and urine biomarkers, to further understand intestinal health

Nanostructured drug delivery systems have attracted considerable attention as potential solutions (18), with nanotechnology offering transformative progress in the development of more efficient and targeted therapeutics (19)

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For genetic ablation study, DIV 7 astrocytes were detached from culture dish surface, electroporated with mCherry-tagged pSicoR vector carrying Scr sequence or shRNA sequences against SIRT2 or ALDH1A1 (shSIRT2 targeting 5-GGAGCATGCCAACATAGATGC-3 or shALDH1A1 targeting 5-TTTCCCACCATTGAGTGCC-3 respectively) and seeded onto coverslips