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Both groups followed a reduced-calorie diet, increased physical activity, and monthly nutritional counseling
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The expression of target genes was analyzed via quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific)
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(in Japanese) Seishin Shinkeigaku Zasshi Psychiatria et Neurologia Japonica 1996
