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For testing the reactivity of compound T13 with GST, T13 (10 M) was reacted with or without GSH (1 mM) and GSTP1 (10 g/mL) at 37C for 1 h, and the product was analyzed by monitoring MRM chromatograms for T13 and T13-GSH

Perhaps future reports of drug induced perturbations in GSH/GST levels should consider whether the biological implications of drug treatment extend further than an enhanced rate of GST mediated catalytic thioether product formation

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Despite being frequently prescribed, MTXs severe multiple toxicities can occasionally limit its therapeutic potential

Mice ( n = 3) anesthetized by intraperitoneal injection of 2.5% pentobarbital sodium (3.5 mL/kg) were scanned on a 3.0 T MR scanner equipped with a 50 mm 65 mm 8-channel phased-array mouse coil (Shanghai Chenguang Medical Technologies Co., Ltd., Shanghai, China)