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If the vial had instead been diluted with only 2mL, the concentration doubles to 10,000mcg/mL, and the same dose would require just 0.25mL (25 units)
doi: 10.1186/s40816-021-00306-z

Brief handling at room temperature is usually unavoidable, but storage at room temperature is not recommended

Comparing surgical interventions for interstitial cystitis: a systematic review

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