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Unbound fractions were washed away, and beads were washed three times with 150 L of Pulldown Wash Buffer (20 mM HEPES pH 7.0, 150 mM KOAc, 2 mM Mg(OAc) 2 , 1 mm dithiothreitol, and 0.1% Tween 20)

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50 mg vial reconstituted with 2 ml BAC water = 25 mg per ml = 0.25 mg per unit

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Special considerations for specific semaglutide formulations Semaglutide with B12 Compounded semaglutide with vitamin B12 (cyanocobalamin) is one of the most common combination formulations