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Briefly, cells were lysed (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5 U/L RNaseOUT, 1 X Roche Protease Inhibitor Cocktail), incubated with Anti-HuR (Molecular Probes, mp21277), sonicated to disrupt RNA, and bound to BSA/Salmon Sperm blocked Magnabeads (Invitrogen) for 16 h at 4 C

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Mice with IRP2 overexpression exhibit motor dysfunction along with increased TfR1, enhanced iron deposition, and upregulation of ferroptosis markers in the SN

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[18] Because these compounds can induce both superficial and internal side effects, they are illegal to use and market in multiple nations