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Reads containing adapters, more than 5% unknown nucleotides or more than 20% low-quality bases were removed to obtain highly clean data

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This concept circumvents mechanisms that have evolved to facilitate species survival, bypasses limitations on molecular transport across the cell wall, and provides a significant departure from traditional, cell-based processes that rely on microscopic cellular reactors. In addition, cell-free systems are inherently distributable through freeze-drying, which allows simple distribution before rehydration at the point-of-use

J Am Chem Soc 98:23392341, 1976