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doi: 10.1007/s00430-021-00727-0 167 LinY.XuY.ZhangZ

PMID: 41253271 J Adv ResGenome-wide CRISPR screen identifies STK11 as a critical regulator of sialic acid clusters important for influenza A virus attachment

For bacterial expression, human DIDO3 SPOC (10471205 aa), SHARP SPOC (34963664 aa), RBM15 SPOC (775960 aa), and SPOCD1 SPOC (8581025 aa) were cloned into pET M11 between NcoI and XhoI for N-terminal His6 fusion
Introduction The gastrointestinal (GI) system plays a central role in human health as the primary site for digestion and nutrient absorption, while also influencing immunity, endocrine signaling, and mental well-being through the gutbrain axis

The EdU assay demonstrated a reduction in cell proliferation following AngII exposure, whereas the AngII+siFasn group showed enhanced proliferation compared to the AngII group ( Figures 6G, H )
