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Finally, 500 ng total RNA was used as a template for the cDNA reaction, which was synthesized using the iScriptcDNA Synthesis Kit (Bio-Rad, Veenendaal, the Netherlands)

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Glucagon is a hormone that is released with low blood sugar, prolonged fasting, exercise, and protein rich meals to prevent blood sugar from dropping too low so inhibiting this with stimulating insulin further reduces blood sugar levels slows the movement of food from the stomach into the small intestine which leads to feeling full faster reducing food intake while eating acts on GLP1 and GIP centers in the brain suppressing appetite which leads to feeling full longer after eating increases insulin sensitivity throughout the entire body Weight loss is an off label use of tirzepatide as it is only FDA approved for glycemic control in patients with type 2 diabetes currently

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