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The SYBR Green 2X PCR mix (Accurate Tech, China) was used for quantitative real-time PCR amplification

10.1111/j.1464-5491.2005.01767.x Diabet Med
This process is characterised by improved oocyte quality and maintenance of primordial follicles

The subjects did not have a current or prior chronic disease or a history of smoking, and they were not currently using any medications
