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In the second case, a 44-year-old woman reported the discontinuation of nail biting after four months of treatment with NAC, dosed at 1000mg twice daily
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After incubation with trypsin, the peptide concentration in samples was estimated using a Micro BCA Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 g peptides was transferred to a new low-bind Eppendorf tube per sample
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