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Recombinant N-terminally MRGS(H) 6 -tagged wild-type and mutant ScGrx7 and HsGrx5 C122S (without their N-terminal targeting sequences 19 ) were purified after lysozyme treatment and sonication by Ni-NTA affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl, and 50 mM sodium phosphate, pH 8.0 11,19,25,42

doi:10.1515/BC.2009.033 Mol Neurobiol