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Due to its reproducibility, quantitative capabilities, and ability to detect a large number of metabolites simultaneously, NMR is well-suited for untargeted metabolomics analyses (29, 30)

In fact, FBP levels correlate with glycolytic flux 53,54 , and FBP is known to act as flux-signalling metabolite in multiple species ranging from bacteria to mammalian cells, transducing metabolic information into diverse cellular responses 55,56,57