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Proper handling is essential: Before Opening: Allow the vial to reach room temperature (1525C) to prevent condensation Briefly centrifuge the vial to collect powder at the bottom Reconstitution Steps: Select solvent: Bacteriostatic water (0.9% benzyl alcohol) for multi-use, sterile water for single-use Calculate volume: Based on desired concentration (e.g., 1 mL for 5 mg = 5 mg/mL) Addition: Slowly inject solvent against the inner wall of the vial, not directly onto the powder Dissolution: Gently swirl (do not vortex) until completely dissolved Visual inspection: Solution should be clear, free of particulates 8.2 Storage Conditions 8.3 Best Practices Protect from light: Store vials in opaque containers Avoid freeze-thaw cycles: Aliquot reconstituted peptide into single-use vials if possible Monitor pH: Some peptides may undergo pH-dependent degradation over time Document: Record reconstitution date, concentration, and storage conditions 9

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Dong X, Biswas A, Suel KE, Jackson LK, Martinez R, Gu H, Chook YM (2009) Structural basis for leucine-rich nuclear export signal recognition by CRM1