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After three 5-min washes in PBS, sections were incubated with the corresponding secondary antibody for 45 min

doi: 10.1016/j.autrev.2009.03.010

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54, 55 Specifically, plasma samples were deproteinated by adding 50% v/v of freshly prepared 10% metaphosphoric acid (MPA), centrifuging at 2000 g for 2 min and then the supernatant stored at 20 C until assaying