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While not required, a calorie deficit with adequate protein (0.7 to 1.0 grams per pound of goal weight) produces best results

Maintaining a healthy GI tract not only enhances nutrient absorption but improves overall well-being [8]

A total of 150 ng of RNA from each sample was used in RT-PCRs performed in a total volume of 25 L using the QIAGEN OneStep RT-PCR Kit (Qiagen, Venlo, The Netherlands)

It is important to note that B12 levels should ideally be measured before commencing replacement therapy, as supplementation rapidly normalises biochemical markers, potentially obscuring the diagnosis

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