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In other studies, prophylactic immunization with the hydatid cyst wall protein bands (~ 27/28 kDa), that showed cross-reactivity with breast cancer patients sera, or crude hydatid cyst wall antigens (laminated layer) caused significant inhibition of 4T1 breast tumor growth, decrease of metastasis, and prolongation of the tumor-bearing mice survival time through a rise in the levels of IL-2, TNF-, IFN-, and IL-4 [51, 181]

doi: 10.1001/jama.300.8.924

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Amplified DNA in the AGM was released into water by heating the tubes at 60 C for 5 min and directly used for the preparation of sequencing libraries using the QIAseq FX DNA Library Kit (Qiagen)