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The fatty acid (e.g., LA, ALA) is stirred at 0 C under oxygen atmosphere with the enzyme in 0.1 M sodium borate buffer pH 10.4

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Adult ceramide synthase 2 (CERS2)-deficient mice exhibit myelin sheath defects, cerebellar degeneration, and hepatocarcinomas
Journal of Clinical Endocrinology and Metabolism , 91(12), 4792-4797
