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For intracellular staining of phospho(p)-mTOR (Fisher Scientific 15549836) and p-S6 (eBioscience 15528216), cells were first stained for extracellular markers, fixed in 2% FA at RT, and stained for p-mTOR and p-S6 diluted 1:200 in saponin 5 g/mL
L., Le Bonniec, B
While the finding that copper potently blocks 1 3 2 GABA A Rs suggests that this metal inhibits fast synaptic, or phasic GABAergic signalling in contrast to zinc, which can only inhibit tonic GABAergic signalling, it is important to note extrasynaptic receptors are sensitive to copper block and, therefore, tonic GABAergic signalling as well
Therefore, the amount of reduced glutathione can be calculated by measuring A412
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