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Figure 5 4 Discussion In view of the inconsistent research results regarding the metabolic characteristics of the microbiota-brain-gut axis in ASD children, we collected morning urine samples to avoid the influence of diet, and adopted the packed-nanofiber solid phase extraction created by our research team to purify and treat biological samples

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However, the precise mechanisms by which SCFAs influence brain physiology and behavior remain to be fully clarified, underscoring a promising avenue for developing novel CNS therapies