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doi: 10.1016/j.jocn.2006.05.020

Reverse transcription was carried out with HiScript III RT SuperMix (Vazyme, Nanjing, Shanghai) following the protocols provided by the manufacturer for qPCR kits

CPSS was diagnosed in all four dogs according to previously described methods 33 All four dogs were fed a standardized commercial diet (Royal Canine, Hepatic) before and during the study, and no medication was given to any of the dogs two weeks prior to the study

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