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They have not been approved by the FDA for medical use

If a biliary colic lasts longer than about three hours or is associated with more severe symptoms such as fever, nausea, vomiting, jaundice, and leukocytosis, the possibility of a gallstone-related complication exists

Before use, the protein was denatured in 6 M urea and supplemented with 2 mM tris(2-carboxyethyl)phosphine (TCEP, Sigma-Aldrich, Solna, Sweden) at pH 7.4 to obtain complete reduction and dissolution of potential aggregates

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