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Each of the concentration levels was prepared in triplicate, and the absorbances were read at 290 nm on a Nanodrop UV Spectrophotometer and recorded accordingly
Click reaction mixture was prepared by sequential addition of 1 volume 10 mM azide-PEG3-biotin (Sigma, 762024), 2 volumes 50 mM CuSO 4 (Sigma, C8027), 2 volumes 50 mM TCEP (Sigma, 68957) and 1 volume 10 mM TBTA (Sigma, 678937)
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