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Polydopamine nanoparticles targeting ferroptosis mitigate intervertebral disc degeneration via reactive oxygen species depletion, iron ions chelation, and GPX4 ubiquitination suppression

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The membrane was washed with Tris-buffered saline (TBS), containing 0.1% Tween 20 (Beyotime Institute of Biotechnology) three times and was subsequently incubated with horesradish peroxidase-labeled secondary antibody (1:2,000