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RNA isolation and real-time RTqPCR Total RNA was isolated from cells or tissues using TRIzol Reagent and the Direct-zol RNA MiniPrep kit (Zymo Research) according to the manufacturers instructions

10.1021/acsami.3c09873 47 LiuT.LiuW.ZhangM.YuW.GaoF.LiC.et al (2018a)

2) intracellular synthesis of arginine from citrulline, which is dependent on citrulline availability

found that fatty acid oxidation regulates the sensitivity of podocytes to palmitic acid and is inversely related to acetyl-CoA carboxylase 2 (ACC2), a key enzyme of FAO [69]

However, GSSG supplementation did no longer lead to reduction of Grx1-roGFP2 in these strains, independent on whether the periplasmic binding protein GsiB or the permease domains GsiC or GsiD were missing, suggesting that Gsi is the exclusive transporter for oxidized glutathione in E