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Cell Viability Assay The cytotoxicity profiles of the indicated formulations were analyzed by making use of the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) microculture tetrazolium viability assay.32 Cells were then seeded in multi-well plates and later incubated for 48 hours with the indicated concentration of Ch-hGH-DOX, Ch-DOX, or DOX (02.5 g/mL)