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M., AL-Qaraawi, H

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Ni-INDIGO beads (20 l) (Cube Biotech, 75105) were added and excess peptide was removed using a PD MidiTRAP G-25 (Cytiva) followed by size-exclusion chromatography (Superdex 75) in 25 mM HEPES, 150 mM NaCl and 1 mM TCEP, pH 7.5

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