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PIP transfer mediated by SMP PDZD-8 [0, 2, 4, 6, or 8 M for PI(4,5)P 2 and 8 M for all other PIPs] was followed by measuring the NBD fluorescence signals of NBD-PH FAPP (0.5 M) at 530 nm (bandwidth 12.5 nm) on excitation at 460 nm (bandwidth 12.5 nm) for 60 min at every 15 s at room temperature

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FMC, fluoromethylcholine

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