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Similarly, for overexpression cell lines, gBlocks (IDT) containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB)

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SOD2 encodes superoxide dismutase two that neutralizes O 2 - generated during oxidative phosphorylation ( SOD2 polymorphism, rs4880 located in exon 2, introduces a T to C substitution at position 2,734, resulting in the SOD2 Ala16Val genotype

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