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Because autophagy may serve as a protective mechanism for CLL cells (36), we then tested CB-839 in combination with the autophagy inhibitor chloroquine at a concentration of 50 M ( Supplemental Figure 5 )

and points represent biological replicates

Coverage statistics from the resulting bam files were calculated using samtools (version 1.9) 89

Based on the optimized basal medium, single-factor experiments were conducted by supplementing different concentrations of histidine (012.5 mg/g), cysteine (04.5 mg/g), and methionine (012.5 mg/g) to evaluate their effects on EGT production

Specifically, autophagy suppresses anoikis induced by the detachment of cancer cells from the extracellular matrix (ECM) and hence promotes metastasis 169