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In conclusion, using a low-carnitine tilapia model, the present study has shown that dietary l-carnitine can be physiologically utilised in fish, and it has the effect of reducing lipid accumulation in the liver

Physical activity increases the body's energy demands, which can accelerate the fat-burning process

Metabolic changes included a reduction of the usage of muscle glycogen (i.e., its carbohydrate stores) during low-intensity exercise (consistent with an increase in lipid utilization) and streamlined glucose metabolism and mitochondrial flux during high-intensity exercise, leading to reduced anaerobic ATP production (which is what leads to lactic acid build-up in muscles)

Furthermore, the protein content was determined in lysed PBMC to generate a normalization factor, using a bicinchoninic acid assay (BC Assay, Uptima, Interchim, Montluon, France)

Acknowledgments The authors thank the staff of Beijing Anzhen Hospital of Capital Medical University and Beijing Institute of Heart, Lung and Blood Vessel Diseases
