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Single- or double-antigen labeling of formalin-fixed and paraffin-embedded 4-m-thick sections by immunocytochemistry (ICC) or immunohistochemistry (IHC) included the following steps: deparaffinization, rehydratation, boiling in EDTA buffer (1 mM EDTA, 0.05% Tween 20, pH 8.0) for 15 min, 1% H 2 O 2 incubation for 15 min and blocking in SuperBlock solution (ScyTek Laboratories, Logan, UT) for 30 min, followed by primary antibody application

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267 DKK1 antibody are involved in the pathogenesis of CKD-MBD, neutralization of DKK1 in CKD-2 mice after renal injury stimulated bone formation rates, corrected the osteodystrophy, and prevented CKD-stimulated vascular calcification

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