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The constructed plasmid, pLdbsh1, was subsequently transformed into AR113 (pLH01) recipient cells via electroporation (Table S2)
[DOI] [PubMed] [Google Scholar] Hockenberry M.J., Hooke M.C., Gregurich M., McCarthy K
Protein was eluted in a single step to 2 M NaCl, 10 mM Bis-Tris pH 6.5, and 10% glycerol
