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Blots were incubated overnight at 4 C with primary antibodies and 2 h with HRP-conjugated secondary antibodies at room temperature

Free radicals are produced naturally during normal body processes and can also result from factors such as: Pollution UV radiation Smoking Poor dietary habits Stress When free radicals accumulate in excess, they can contribute to oxidative stress, which may affect normal cell function over time

Wagner et al

(Phila.) 2012;50(2):9198