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GSH depletion causes oxidative stress and lipid peroxidation To evaluate the downstream effects of GSH depletion, we measured soluble ROS in RPE cells treated with Cys 2 starvation, BSO, or erastin by flow cytometry using the fluorescent probe H 2 DCFDA

Y., Nguyen P

( A ) Diagram showing the design of the BRET assay to measure binding of Nluc-fused peptides to active (QL mutant) or inactive (WT) G subunits fused to YFP

Possible Side Effects Side effects are rare when administered correctly but may include: Mild redness Temporary discomfort Slight nausea (rare) Allergic reactions (extremely rare) These typically resolve quickly under expert care

U., Shah S