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RT-PCR An RNeasy kit (Qiagen, 74104) was used to extract total RNA from mice hippocampal tissue, and then the Transcriptor High Fidelity cDNA synthesis kit (Roche, 5081963001) was used to reverse transcribe the RNA into cDNA

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Zhang Y, Xia M, Jin K, Wang S, Wei H, Fan C, Wu Y, Li X, Li X, Li G, et al

The costs of implementation are modest compared to the current economic burden of delayed diagnosis, which includes excess healthcare utilization, productivity losses, and reduced quality of life

Its the thinking and the preoccupation, which can show up alongside physical hunger, alongside a craving, or on its own