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[DOI] [PMC free article] [PubMed] [Google Scholar] 143.Li B., Lei S., Xiong S., Chen S., Zhang Z
The two viruses for Cre recombinase and small guide RNA (sgRNA) were injected into the prelimbic cortex of CRISPR/CAS9-EGFP mice
T., Johnson, W
In the next step slides were incubated in cold lysis solution (150 mM NaCl, 30 mM EDTA, 10 mM Tris-HCl, 0.1% N-lauroyl sarcosine, pH 8.3) for 20 min and washed in TBE buffer, pH 8.3 for 5 min
Inhibition of Ca(2+) signaling by Mycobacterium tuberculosis is associated with reduced phagosome-lysosome fusion and increased survival within human macrophages
