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After incubation, cells were washed three times using DPBS (1x) (Sigma Aldrich

an overly acidic environment may lead to the dissociation of the copper ion from the peptide, compromising the efficacy and color of the product

Additionally, damage-associated molecular patterns (DAMPs) interacting with Toll-like receptor 4 (TLR4) synergistically phosphorylate and degrade inhibitor of kappa B alpha (IB), thereby promoting the nuclear translocation of the p50/rela dimer and initiating the transcription of pro-inflammatory factors

doi: 10.1523/JNEUROSCI.5215-08.2009

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