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Description
K.OgunladeE
The slides were incubated with fluorescently labeled secondary antibodies (Alexa Fluor 488-conjugated goat anti-mice immunoglobulin G, Beyotime, China or Alexa Fluor 488-conjugated goat anti-mice immunoglobulin G, Beyotime, China) for 2 h at 37C and 4,6-diamidino-2-phenylindole (DAPI) (#c-1002, Beijing, China) for half an hour at room temperature, and finally, the slides were sealed with antifluorescent quencher and nail polish
Viral supernatant harvested from transfected cells was sterile filtered and stored at -70C until required
Overall, preclinical studies have shown that NAD + and mitochondrial metabolism play a crucial and intertwined role in aging and age-associated diseases, as well as being possible therapeutic targets for ameliorating these conditions
Insulin resistance is one of the leading causes of aging, and improving insulin sensitivity may help slow down the aging process
