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Reconstituting peptides is the process of dissolving a freeze-dried peptide powder in bacteriostatic water or sterile water to prepare it for injection, and doing it correctly protects both potency and safety

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The N-terminal HA-tagged wild type and single-lysine mutant ubiquitin constructs were generously provided by Dr

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When required, media were supplemented with the relevant antibiotics at the following concentrations: 25 g mL -1 kanamycin, 25 g mL -1 erythromycin for E