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Post-operatively the animals were housed individually in an American Accreditation for Laboratory Animal the water maze consisted of a circular tank painted black (diameter: 1.6 m

The tissues were homogenized in 500 l of lysis buffer (cat# RB4475, BioBasic, Canada) supplemented with 1% protease inhibitor cocktail set I (cat# 539131, Sigma-Aldrich, USA)

Cysteine deficiency impairs glutathionylation of succinate dehydrogenase A (SDHA), thereby impairing the activity of the electron transport chain complex II (ETC II), inhibiting oxidative phosphorylation, reducing ATP levels, and leading to LSC death (Jones et al

Magnesium promotes root growth and increases aluminum tolerance via modulation of nitric oxide production in Arabidopsis

However, much of this literature is limited by small sample sizes, observational designs, heterogeneous phase definitions, and incomplete control of diet, physical activity, and ovulation verification, which constrains causal inference