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However, observations from in vivo studies complicate the picture even further

Selected Research Highlights NAD Studied extensively in relation to mitochondrial function, cellular energy transfer, and metabolic-stress responses

After a wash step, cells were incubated with fixation/permeabilization concentrate for 30 minutes to fix the surface and permeabilize cells for intracellular staining

| Concentration | BAC Water | Doses at 1.0mg | Doses at 2.4mg | |---------------|-----------|----------------|----------------| | 5mg/mL | 2.0mL | 10 doses | ~4 doses | | 2.5mg/mL | 4.0mL | 10 doses | ~4 doses | **Best practice:** 2.0mL BAC water gives a clean 5mg/mL concentration

Clinic frameworks typically follow a similar pattern, recommending a break of roughly equal length