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doi: 10.1128/MCB.24.19.8477-8486.2004

Three-micron sections were prepared and mounted on slides, followed by deparaffinization and immunofluorescent staining according to a standard protocol as follows: For deparaffinization, the paraffin-embedded sections were placed at 60 C for at least 30 min, incubated twice in xylene (5 minutes each), and then transferred into 100% ethanol (2 minutes), 100% ethanol (2 minutes), 90% ethanol (1 minute), 80% ethanol (1 minute), and 70% ethanol (1 minute) at room temperature

Researchers monitor temperature as an early indicator of disease and response in animals and humans

egl-9(sa307) animals with ethyl methanesulfonate (EMS) as described previously 51

doi: 10.1038/s41467-021-22624-z