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Though functional roles of the VGF peptides detected here remain to be established, consistent changes in VGF abundance across proteoforms between diagnostic classes in the present study, as well as previous literature, suggest clear roles of VGF peptides in cognitive functioning 15

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Despite this, substitution of a polar uncharged side chain (Ser or Thr, -CH 2 OH or -CH 2 (OH)CH 3 ) with a polar negatively charged (Asp, -CH 2 COOH) side chain mimicking phosphorylation is still sufficient to disrupt Golgi localization and fragmentation, implying the 3D mutant likely repulses membranes

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