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Protein staining in the gel was performed using a Coomassie staining solution (Ammonium sulfate 10%phosphoric acid 10%Coomassie G250 0.12% and ethanol 20%) overnight with shaking at room temperature followed by destaining with distilled H 2 O

P21 is BBB permeable with a plasma half-life exceeding six hours, giving it sustained access to brain tissue

Spence JW

Protein Expression and Purification The recombinant mutant plasmid was transformed to Escherichia coli strain M15

[15] [16] Pyruvate dehydrogenase complex Once in the mitochondrial matrix, pyruvate is mostly oxidized to carbon dioxide in order to support ATP production