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Briefly, independent cultures of Agrobacterium carrying pTRV1 or pTRV2-based constructs were resuspended in 10 mM MgCl 2 , 10 mM MES (pH 5.6) and 150 M acetosyringone at OD 600 = 0.8, and incubated for 2 h at 28 C in the dark

A possible copperproline complex from the roots ofArmeria maritima

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For all individuals who have the gene abnormality, the absorption of B-complex vitamins is either largely inefficient or lacking altogether